时间：
地点： 环保楼204室
联系人：资源环境学院
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Qing X. Li, Ph.D., Professor
Department of Molecular Biosciences and Bioengineering,
Phone: +1-808-956-2011; FAX: +1-808-956-3542 or 5037; E-mail: qingl@hawaii.edu
Education
B.S. in Agriculture, 1982,
Ph.D. in Agricultural and Environmental Chemistry, 1990,
Post-doctoral fellow, 1991-1994,
Research
Research in my laboratory focuses on environmental biochemistry and biotechnology. Current research projects are in areas of proteomics, metabolomics, bioremediation, marine pollution, and immunochemistry and antibody-based assays.
Selected Publications (out of 120)
Pellequer, J.L.; Zhao, B.; Kao, H.-I.; Bell, C.W.; Karu, A.E.; Li, K.; Li, Q.X.; Roberts, V.A. 2000. Stabilization of bound polycyclic aromatic hydrocarbons by a p-cation interaction. J. Mol. Biology. 302(3):691-699.
Keum, Y.-S.; Li, Q.X. 2004. Copper dissociation as a mechanism of fungal laccase denaturation by humic acid. Appl. Microbiol. Biotech. 64:588-592.
Hou, S.; Saw, J.; Lee, K.S.; Freitas, T.A.; Belisle, C.; Kawarabayasi, Y.; Donachie, S.P.; Galiperin, M.Y.; Koonin, E.V.; Makarova, K.S.; Omelchenko, M.V.; Sorokin, A.; Wolf, Y.I.; Li, Q.X.; Keum, Y.S.; Campbell, S.; Denery, J.; Aizawa, S.-I.; Shibata, S.; Malahoff, A.; Alam, M. 2004. Genome sequence of the deep-sea γ-Proteobacterium Idiomarina Ioihiensis reveals amino acid fermentation as source of carbon and energy. Proc Natl Acad Sci
Keum, Y.-S.; Li, Q.X. 2005. Reductive debromination of polybrominated diphenyl ethers by zero-valent Iron. Environ. Sci. Technol. 39(7):2280-2286.
Delanoy, G.; Li, Q.X.; Yu, J. 2005. Activity and stability of laccase in conjugation with chitosan. Int’l J. of Biological Macromolecules. 35(1-2): 89-95.
Shelver, W.L.; Kim, H.-J.; Li, Q.X. 2005. Development of monoclonal antibody based ELISA for the b-adrenergic agonist zilpaterol. J. Agric. Food Chem. 53: 3273-3280.
Pelleguer, J.-L.; Chen, S.-W. W.; Karu, A.E.; Li, Q.X.; Roberts, V.A. 2005. Structural basis for preferential binding of non-ortho-substituted polychlorinated biphenyls by the monoclonal antibody S2B1. J. Mol. Recog. 18:282-294.
Parrotta, C.D.; Rubio, F.; Li, Q.X.; Shelver, W.L. 2005. Development of a sensitive magnetic particle immunoassay for polybrominated diphenyl ethers. Organohalogen Compounds. 67: 27-30.
Shelver, W.L.; Keum, Y.-S.; Li, Q.X.; Elliott, C.T. 2005. Development of an immunobiosensor assay for the beta-adrenergic agonist zilpaterol. J. Food & Agric. Immunol. 16(3): 199-211.
Cho, I.-K.; Chang, C.L.; Li, Q.X. 2005. Nicotinamide in relation to dietary nicotinic acid and nine other vitamins and larval development of Ceratitis capitata (Diptera: Tephritidae). J. Agric. Food Chem. 53:7307-7311.
Harada, R.M.; Campbell, S.; Li, Q.X. 2006. Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll,
Denery, R.J.; Cooney, J.M.; Li, Q.X. 2006. Metabolic profiling to reflect gene expression in Streptomyces tenjimariensis. Industrial Biotechnology. 2(1):51-54.
Seo, J.S., Keum, Y.S., Cho, I.K., Li, Q.X. 2006. Degradation of dibenzothiophene and carbazole by Arthrobacter sp. P1-1. Int. Biodeterioration & Biodeg. 58:36-43.
Keum, Y.S., Seo, J.S., Hu, Y., Li, Q.X. 2006. Degradation pathways of phenanthrene by Sinorhizobium sp. C4. Appl. Microbiol. Biotechnol. 71:935-941.
Seo, J.S., Keum, Y.S., Hu, Y., Lee, S.E., Li, Q.X. 2006. Phenanthrene degradation in Arthrobacter sp. P1-1: Initial 1,2-, 3,4- and 9,10-dioxygenation, and meta- and ortho-cleavages of naphthalene-1,2-diol after its formation from naphthalene-1,2-dicarboxylic acid and hydroxyl naphthoic acids. Chemosphere 65:2388-2394.
Lee, S.-E.; Li, Q.X.; Yu, J. 2006. Proteomic responses to formic acid on Ralstonia eutropha. Proteomics 6:4259-4268.
Lee, S-E; Seo, J.S.; Keum, Y.-S.; Li, Q.X. 2007. Fluoranthene metabolism and associated proteins in Mycobacterium sp. JS14. Proteomics. Accepted.
Seo, J.S., Keum, Y.S., Hu, Y., Lee, S.E., Li, Q.X. 2007. Degradation of phenanthrene by Burkholderia sp. C3: initial 1,2- and 3,4-dioxygenation and meta- and ortho-cleavage of naphthalene-1,2-diol. Biodegradation. In press. DOI 10.1007/s10532-006-9048-8.
Research Facility:There are about 4000 square feet of laboratory space, ten standard offices. This laboratory is suitably equipped for research in environmental biochemistry and biotechnology. Major instruments in this laboratory include: one Varian Saturn 2000 ion trap GC-MS; one Varian GC-1200 Quadrupole MS; one UltimateTM nano 2D LC system interfaced to Bruker esquireHCTplus ion trap nanoelectrospray MS; one Agilent 1100 LC-MSD (SL-ESI, APCI); two Isco supercritical fluid extractor; two Dionex accelerated solvent extractors; one PTI spectrofluorometer; one Dionex BioLC HPLC and one Shimadzu LC-10S HPLC with UV/VIS, diode array and fluorescence detectors; four GCs with ECD, FID, NPD, PFD detectors; one Molecular Devices 96-well microplate reader; one Spidyne KinExA; one Brandel cell harvester; one Sorvall RC5C centrifuge; one Beckman L8-M ultracentrifuge; one 96-capillaries high through put MegaBace DNA Sequencing System 1000; BioRad denaturing gradient gel electrophoresis (DGGE) system; DNA thermal cyclers; gel electrophoresis apparatuses; one Varian Gary 50 Bio UV-visible spectrophotometer; one Thermo Electron Corp Nicolet 380 FT-IR; high- and low-speed preparative microcentrifuges; water baths and incubators. We are now in the process of purchasing a MOLDI-TOF/TOF and a Q-TOF.
We have the dual-processor license for local MASCOT™ (Matrix Science) server and BioTools™ to process proteomic data and protein analysis, the Metabolic Profiler™ integrating metabolism studies and pharmaco-metabolomics, and CAChe Worksystem Pro (Version 5.0, Fujitsu) and Molecular Modeling Pro (Version
